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Product Details
Source
Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
3H8B10
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
Purified Human CD16a Protein
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
This product is a specific antibody specifically reacts with CD16a protein. ACRObiosystems offers two anti-CD16 antibodies (clones 3H8B10 and 3G8) that target distinct epitopes on CD16.
Application
Flow Cytometry (Evaluation of the expression of CD16a on Human cells).
Recommended Dilution
1:20
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-Flow Cytometry
Flow cytometric analysis of human peripheral blood lymphocytes respectively staining with PE-Labeled Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) (Cat. No. CDA-PFM785) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and FITC anti-human CD56 Antibody, compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Flow cytometric analysis of Human peripheral blood mononuclear cell respectively staining with PE-Labeled Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) (Cat. No. CDA-PFM785) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
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Preguntas Frecuentes
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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