COA
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Spike,S protein,Spike glycoprotein,S glycoprotein
MERS Spike protein trimer, His Tag (SPN-M52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K9N5Q8-1). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Tyr 18
Request for sequence
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Its been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
