1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
3. Comprehensive application data to support assay development and validation
4. Full tracible record, stringent quality control and validated cell passage stability
5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
6. Global commercial license assistance whenever regulatory filing is required

Description

The Jurkat/human TRBV9 & Luc Stable Cell Line was engineered to express the exogenous TCRβ chain harboring the human TRBV9 variable domain (NCBI: NG_001333.2) following knockout of the endogenous TCRβ chain. This reconstituted TRBV9-containing TCRβ chain pairs with the endogenous TCRα chain to reform the TCRαβ heterodimer, which subsequently assemble with the endogenous CD3 to enable cell surface expression of the TCR/CD3 complex. Surface expression of human TRBV9 was confirmed by flow cytometry. The endogenous TCRβ knockout was confirmed by genomic sequencing. This cell was also engineered to express the firefly luciferase reporter. The luciferase activity was confirmed by the detection of luminescence signal.

Application

• Useful for cell-based TRBV9 binding assay

• Used as target cells for luminescence-based cytotoxicity evaluation

Growth Properties

Suspension

Selection Marker

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

Complete Growth Medium

RPMI-1640 + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process