Human TRAIL R4 / TNFRSF10D Protein, Fc Tag (MALS verified)

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TR4-H5253-100ug
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TR4-H5253-1mg
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TR4-H5253
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Synonyms

TNFRSF10D, CD264, TRAILR4, DCR2, TRUNDD

Source

Human TRAIL R4 Protein, Fc Tag (TR4-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 56 - His 211 (Accession # Q9UBN6).

Predicted N-terminus: Ala 56

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 43.2 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D) is also known as Decoy receptor 2 (DcR2), TNF-related apoptosis-inducing ligand receptor 4 (TRAIL receptor 4 or TRAIL-R4), CD264,which is the single-pass type I membrane protein. TNFRSF10D is receptor for the cytotoxic ligand TRAIL, which contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis.