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Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Human TL1A Protein, His Tag (TLA-H5246) is expressed from human 293 cells (HEK293). It contains AA Leu 72- Leu 251 (Accession # O95150-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 26 kDa and 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human TL1A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TL1A Protein, His Tag (Cat. No. TLA-H5246) is more than 90% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human TL1A Protein, His Tag (Cat. No. TLA-H5246) with a linear range of 8-125 ng/mL (QC tested).
Protocol
Human TL1A Protein, His Tag (Cat. No. TLA-H5246) induced NF-κB Reporter activity in Jurkat Cell. The EC50 value of this effect is 1.163 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-TL1A antibody captured on Protein A Chip can bind Human TL1A Protein, His Tag (Cat. No. TLA-H5246) with an affinity constant of 0.293 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human DcR3, Fc Tag (Cat. No. TNB-H5255) captured on Protein A Chip can bind Human TL1A Protein, His Tag (Cat. No. TLA-H5246) with an affinity constant of 0.497 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 15
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
18 Details
- Highest Development Stage：
Phase 3 Clinical
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