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Mucin 1, MUC1, CD227, EMA, H23AG, KL-6, MAM6, MUC-1, SEC, MUC-1, X, MUC1, ZD, PEM, PEMT, PUM, CA15-3, Episialin
Biotinylated Human Mucin-1 (890-1158), His,Avitag (MU1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ser 890 - Gly 1158 (Accession # P15941-1).
Predicted N-terminus: Ser 890 (partial α chain) & Ser 1098 (partial β chain)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 21.3 kDa (partial α chain) and 10.2 kDa (partial β chain).The protein migrates as 45-70 kDa (partial glycosylated α chain), 11 kDa and 14-15 kDa (partial glycosylated β chain) under reducing (R) condition (SDS-PAGE).
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.