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Product Details
Synonyms
MIC-A
Source
Human MICA (24-309) Protein, Fc Tag (MIA-H5254) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Thr 309 (Accession # Q29983-1).
Predicted N-terminus: Glu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 59.4 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human MICA (24-309) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human MICA (24-309) Protein, Fc Tag (Cat. No. MIA-H5254) is more than 85% and the molecular weight of this protein is around 140-165 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human MICA (24-309) Protein, Fc Tag (Cat. No. MIA-H5254) at 1 μg/mL (100 μL/well) can bind Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) with a linear range of 0.01-0.156 μg/mL (QC tested).
Protocol
Immobilized Human NKG2D, His Tag (Cat. No. NKD-H5245) at 2 μg/mL (100 μL/well) can bind Human MICA (24-309) Protein, Fc Tag (Cat. No. MIA-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
MHC class I polypeptide-related sequence A
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 1 Clinical
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