Biotinylated Human Langerin Protein, Avitag™,His Tag

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LAN-H82Q3-20ug
$350.00
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LAN-H82Q3-200ug
$1,380.00
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LAN-H82Q3
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Synonyms

CLEC4K, CD207, Langerin

Source

Biotinylated Human Langerin Protein, Avitag,His Tag (LAN-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Pro 65 - Pro 328 (Accession # Q9UJ71.2).

Predicted N-terminus: Gly

Molecular Characterization

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 33.4 kDa. The protein migrates as 38-40 kDa and 42-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Langerin (CD207) is a crucial C-type lectin receptor (CLR) primarily expressed by Langerhans cells (LCs) in the epidermis. It functions as a pattern recognition receptor (PRR), utilizing its extracellular domain to specifically bind carbohydrate structures on pathogens (e.g., mannose, fucose). Uniquely associated with Birbeck granules, Langerin mediates efficient antigen internalization and processing, thereby enabling LCs to initiate adaptive immune responses and restrict certain infections. Its distinct expression also serves as a key diagnostic marker for Langerhans Cell Histiocytosis (LCH).