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Biotinylated Human Langerin Protein, Avitag™,His Tag

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Cat. No. / Size
Price
Qty
LAN-H82Q3-20ug
$350.00
LAN-H82Q3-200ug
$1380.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    CLEC4K, CD207, Langerin

  • Source

    Biotinylated Human Langerin Protein, Avitag,His Tag (LAN-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Pro 65 - Pro 328 (Accession # Q9UJ71.2).

    Predicted N-terminus: Gly

    Request for sequence
  • Molecular Characterization

    Langerin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 33.4 kDa. The protein migrates as 38-40 kDa and 42-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Langerin SDS-PAGE

    Biotinylated Human Langerin Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

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Background

Langerin (CD207) is a crucial C-type lectin receptor (CLR) primarily expressed by Langerhans cells (LCs) in the epidermis. It functions as a pattern recognition receptor (PRR), utilizing its extracellular domain to specifically bind carbohydrate structures on pathogens (e.g., mannose, fucose). Uniquely associated with Birbeck granules, Langerin mediates efficient antigen internalization and processing, thereby enabling LCs to initiate adaptive immune responses and restrict certain infections. Its distinct expression also serves as a key diagnostic marker for Langerhans Cell Histiocytosis (LCH).

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