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IL12, p70, Interleukin-12
Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 38.3 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-12A is fused with flag tag at the C-terminus. The protein migrates as 32-35 kDa (IL12A) and 45-50 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.