COA
- Product Details
- Performance Data
- Reviews & Citations
- Resources
1/2
GPRC5D, G-protein coupled receptor family C group 5 member D
Nanodisc Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (GPD-H82D7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2).
Predicted N-terminus: Asp
This protein carries a Flag tag at the N-terminus, followed by a polyhistidine tag and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 40.1 kDa & 24.7 kDa.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H81Q5) is sold separately and not included in protein, you can follow the link for product information.
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.