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Product Details
Synonyms
gp130 & OSMR
Source
Human gp130 & OSMR Heterodimer Protein, His Tag (GPR-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 & Glu 28 - Met 740 (Accession # P40189-1 & Q99650-1).
Predicted N-terminus: Glu 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 151.8 kDa. The protein migrates as 170-230 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human gp130 & OSMR Heterodimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human gp130 & OSMR Heterodimer Protein, His Tag (Cat. No. GPR-H52H3) is more than 90% and the molecular weight of this protein is around 170-230 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human Oncostatin M (OSM) (26-221) Protein, premium grade (Cat. No. OSM-H5214) at 5 μg/mL (100 μL/well) can bind Human gp130 & OSMR Heterodimer Protein, His Tag (Cat. No. GPR-H52H3) with a linear range of 10-156 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human gp130 & OSMR Heterodimer Protein, His Tag (Cat. No. GPR-H52H3) immobilized on CM5 Chip can bind Human Oncostatin M (OSM) (26-221) Protein, premium grade (Cat. No. OSM-H5214) with an affinity constant of 5.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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