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Product Details
Synonyms
TNFSF18, AITRL, TL6, hGITRL, GITR Ligand
Source
Human GITR Ligand Protein, His Tag, active trimer (GIL-H524a) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human GITR Ligand Protein, His Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human GITR Ligand Protein, His Tag, active trimer (Cat. No. GIL-H524a) is more than 90% and the molecular weight of this protein is around 45-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human GITR, Fc Tag (Cat. No. GIR-H5254) at 5 μg/mL (100 μL/well) can bind Human GITR Ligand Protein, His Tag, active trimer (Cat. No. GIL-H524a) with a linear range of 1-20 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 18
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
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