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COADS MSDS

Biotinylated Canine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™&Tag Free (MALS & SPR verified)

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COADS MSDS
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FCM-C82W9-25ug
$455.00
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FCM-C82W9-200ug
$1,795.00
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FCM-C82W9
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

FcRn, FCGRT & B2M

Source

Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (FCM-C82W9) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Pro 298 & Val 28 - Asn 125 (Accession # XP_038512242.1 & NP_001271408.1).

Predicted N-terminus: Ala 23 | Val 28

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 28.3 kDa (FCGRT) & 11.5 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The protein migrates as 38-40 kDa (FCGRT) and＜14.4 kDa (B2M) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.