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Human FASN Protein, His Tag (active enzyme)

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Cat. No. / Size
Price
Qty
FAN-H5143-100ug
$350.00
FAN-H5143-1mg
$2310.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    FAS, OA-519, SDR27X1

  • Source

    Human FASN Protein, His Tag (FAN-H5143) is expressed from E. coli cells. It contains AA Leu 2200 - Gly 2511 (Accession # NP_004095-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    FASN Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 58.3 kDa. The protein migrates as 34-39 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.9 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FASN SDS-PAGE

    Human FASN Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity

    Measured by its ability to hydrolyze 4-methylumbelliferyl heptanoate. The specific activity is >3 pmol/min/μg (QC tested).

    Protocol

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Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

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