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Biotinylated Human CLEC4M Protein, Avitag™,His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CLM-H82Q3-25ug
$380.00
CLM-H82Q3-200ug
$1509.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    CLEC4M, CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, L-SIGN, LSIGN

  • Source

    Biotinylated Human CLEC4M Protein, Avitag,His Tag (CLM-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Gln 71 - Glu 399 (Accession # Q9H2X3).

    Predicted N-terminus: Gly

    Request for sequence
  • Molecular Characterization

    CLEC4M Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 41.3 kDa. The protein migrates as 42-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a tetramer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CLEC4M SDS-PAGE

    Biotinylated Human CLEC4M Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CLEC4M SEC-MALS

    The purity of Biotinylated Human CLEC4M Protein, Avitag,His Tag (Cat. No. CLM-H82Q3) is more than 85% and the molecular weight of this protein is around 165-185 kDa verified by SEC-MALS.

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Background

CLEC4M encodes a C-type lectin receptor crucially involved in cell adhesion and diverse pathogen recognition. This receptor notably binds a wide array of evolutionarily divergent and clinically significant pathogens, including Mycobacterium tuberculosis, as well as viruses such as Ebola, Hepatitis C (HCV), HIV-1, Influenza A, West Nile virus, and the SARS-CoV acute respiratory syndrome coronavirus. Structurally, the protein is organized into four distinct domains: an N-terminal cytoplasmic domain implicated in internalization, a transmembrane region, a flexible tandem-repeat neck domain of variable length, and a C-terminal carbohydrate recognition domain (CRD). CLEC4M shares close sequence and functional homology with the neighboring gene CD209 (DC-SIGN), though they differ in specific viral recognition profiles and tissue expression patterns. CLEC4M exhibits high expression in the endothelial cells of the liver, lymph node, and placenta, and crucially, polymorphisms within its tandem-repeat neck domain have been associated with resistance to SARS infection.

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