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Product Details
Synonyms
CD6, TP120, T12
Source
Cynomolgus CD6 Protein, His Tag (CD6-C52H3) is expressed from human 293 cells (HEK293). It contains AA His 18 - Leu 399 (Accession # XP_005577746.1).
Predicted N-terminus: His 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 42.8 kDa. The protein migrates as 55-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus CD6 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD6 Protein, His Tag (Cat. No. CD6-C52H3) is more than 95% and the molecular weight of this protein is around 80-100 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human ALCAM, Fc Tag (Cat. No. CD6-H5259) at 5 μg/mL (100 μL/well) can bind Cynomolgus CD6 Protein, His Tag (Cat. No. CD6-C52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell differentiation antigen CD6
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Approved
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