Biotinylated Human BLAME / SLAMF8 Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
BLE-H82E4-25ug
$360.00
BLE-H82E4-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    BLAME, SLAMF8, CD353

  • Source

    Biotinylated Human BLAME, His,Avitag (BLE-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Asp 233 (Accession # Q9P0V8-1).

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  • Molecular Characterization

    BLAME Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    BLAME SDS-PAGE

    Biotinylated Human BLAME, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.

  • Bioactivity-ELISA

     BLAME ELISA

    Immobilized Human BLAME, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BLAME, His,Avitag (Cat. No. BLE-H82E4) with a linear range of 1-31 ng/mL (QC tested).

    Protocol

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Background

BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8(SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (MΦs) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.

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