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Product Details
Synonyms
ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1
Source
Biotinylated Human ASGR1 Protein, His,Avitag (GS1-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Gln 62 - Leu 291 (Accession # P07306-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 33.4 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human ASGR1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Human ASGR1 Protein, His,Avitag (Cat. No. GS1-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Anti-Human ASGR1 antibody-4G5, Mouse IgG2b|Mouse Kappa with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human ASGR1 Protein, His,Avitag (Cat. No. GS1-H82Q3) on SA Biosensor, can bind SARS-CoV-2 , S1 protein, His tag with an affinity constant of 22.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
N***i2024-11-25
LikesI recently purchased the hASGPR protein from Acro Biosystems for use in my SPR (surface plasmon resonance) experiments, and I am thrilled with the results! The protein quality was excellent, and it delivered reliable and reproducible data, which significantly boosted the confidence in my study. The purity and functional activity of the hASGPR were clearly top-notch, making it easy to work with and yielding strong, clean sensorgrams. I am particularly impressed with how well it performed in terms of binding affinity and kinetics analysis, which was a critical aspect of my research. Shipping was also quick, and the product was well-packaged to maintain its integrity. Overall, I highly recommend this product to anyone conducting SPR experiments requiring hASGPR. Great product and great experience!
Background
Recent Advances
Drug Development Progress- English Name：
Asialoglycoprotein receptor 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 2 Clinical
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