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Product Details
Synonyms
ANGPTL7, CDT6
Source
Human ANGPTL7 Protein, His Tag (AN7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 48-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human ANGPTL7 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ANGPTL7 Protein, His Tag (Cat. No. AN7-H52H3) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
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Bioactivity-SPR
Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) immobilized on CM5 Chip can bind Human ANGPTL7 Protein, His Tag (Cat. No. AN7-H52H3) with an affinity constant of 0.792 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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