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Human Angiogenin / RNASE5 Protein, His Tag (SPR verified)

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Cat. No. / Size
Price
Qty
ANN-H5149-100ug
$390.00
ANN-H5149-1mg (250ug X 4)
$2885.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    ALS9, ANG, Angiogenin, angiogenin, ribonuclease, RNase A family, 5, EC 3.1.27, EC:3.1.27.-

  • Source

    Human Angiogenin, His Tag (ANN-H5149) is expressed from E. coli cells. It contains AA Gln 25 - Pro 147 (Accession # P03950-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Angiogenin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 16.0 kDa. The protein migrates as 12 kDa and 17-18 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Angiogenin SDS-PAGE

    Human Angiogenin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-SPR

     Angiogenin SPR

    Human Angiogenin, His Tag (Cat. No. ANN-H5149) immobilized on CM5 Chip can bind Human Plexin B2, His Tag (Cat. No. PL2-H52H3) with an affinity constant of 17.5 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo

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Drug Development Progress
  • English Name:

    Angiogenin

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Preclinical

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