Product Details
Synonyms
IAP
Source
Biotinylated Human ALPI Protein, His,Avitag (ALI-H82E3) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Asp 503 (Accession # P09923-1).
Predicted N-terminus: Val 20
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 56.0 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Biotinylated Human ALPI Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Human ALPI Protein, His,Avitag (Cat. No. ALI-H82E3) is more than 90% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated Human ALPI Protein, His,Avitag (Cat. No. ALI-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Anti-ALPP/ALPG/ALPI Polyclonal Antibody (QC tested).
Protocol
Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >4,000 pmol/min/µg (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Intestinal alkaline phosphatase
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
1 Details
- Highest Development Stage:
Phase 1 Clinical



















