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Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) at 1.6 μg/mL (0.16 μg of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Goat anti-Rabbit IgG (H+L), PE. PE signal was used to evaluate the binding activity (QC tested).
Non-specificity of Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC anti-CD3 antibody and Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (0.16 μg of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
The purity of Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
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