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> cutting-Edge TR-FRET Solutions
TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) is a detection technology that combines Fluorescence Resonance Energy Transfer (FRET) with Time-Resolved Fluorescence (TRF) measurement. It relies on the distance between donor and acceptor fluorophores—when they are brought close together, energy transfer occurs, generating a measurable signal. The time-resolved component introduces a delay in signal detection, effectively minimizing interference from autofluorescence and scattered excitation light.
By integrating the strengths of both FRET and TRF, TR-FRET offers high sensitivity, ease of use, and reliable results. It is well-suited for high-throughput screening and widely applicable in protein quantification, signaling pathway analysis, disease mechanism studies, and more—making it a valuable tool in modern drug discovery and development.
Fluorescence Resonance Energy Transfer (FRET): When molecules are not bound, the donor and acceptor remain far apart—typically more than 10 nm—so the excited donor cannot effectively transfer energy to the acceptor. As a result, only the donor emission peak (620 nm) is observed; When molecular binding occurs, the donor and acceptor are brought into close distance—usually less than 10 nm—allowing energy transfer from the excited donor to the acceptor via FRET. This results in emission from the acceptor, producing a detectable signal at 665 nm.
Time-Resolved Fluorescence (TRF) Detection: After the excitation pulse ends, fluorescence signals from the donor and acceptor are measured following a short delay—typically between 50 and 150 microseconds. This delay effectively eliminates interference from nonspecific background fluorescence. By analyzing the ratio of fluorescence intensities, TRF enables accurate assessment of molecular interactions, including binding strength, affinity, and kinetics.
High Throughput– Homogeneous assay with no washing steps
High Sensitivity– High signal-to-noise ratio with minimal background interference
Low Sample Consumption– Small reaction volumes save both samples and reagents
Excellent Reproducibility– Simple workflow minimizes experimental variability
Leveraging its deep expertise in protein and antibody development, ACROBiosystems has successfully built a high-performance TR-FRET technology platform, delivering one-stop, cutting-edge solutions for biopharmaceutical research. We offer TR-FRET kits for diverse applications, highly compatible universal tools, and customized development services to help clients rapidly establish sensitive and reliable detection systems. Our solutions significantly enhance experimental efficiency—from target validation to preclinical studies—accelerating the development of innovative therapeutics.
High-quality core raw materials
With the well-established protein and antibody development platform, ACRO provides high-quality raw materials featuring native structure, intact conformation, high biological activity, and stringent quality control—ensuring consistent performance across every batch of reagents.
More richer product matrix
A diverse range of product types covering various popular target, whether you're conducting high-throughput drug screening, protein quantification, or signaling pathway research, you’ll find the TR-FRET solution that best fits your needs.
More assured product quality
Each TR-FRET kit undergoes rigorous quality control and multi-dimensional validation. Systematically verified through established methodologies and tested in real drug development scenarios, our kits deliver reliable results you can trust.
Exceptional value for money
ACRO thoughtfully accounts for reagent loss during dilution and pipetting by providing overfilled packaging. This ensures consistent supply and long-term research support—all at a highly competitive price.
Flexible purchasing options
To meet diverse experimental needs, you can choose complete kits or toolbox for greater flexibility and cost efficiency. We also offer TR-FRET kit development service tailored to your specific requirements.
Protein-Protein Interaction Kit (PPI)
Toolbox
The kit is suitable for the detection of FcRn inhibitors. It shows that both Efgartigimod and its biosimilar, Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) His Tag (Cat. No. IG1-H52H8) exhibit good inhibitory activity in this TR-FRET competition assay.
| Molecule | Cat. No. | Product Description |
|---|---|---|
| FcRn (FCGRT & B2M) | FRT-01 | Human FcRn binding Kit (TR-FRET) |
| TL1A-DR3 | FRT-02 | Human TL1A-DR3 inhibition Kit (TR-FRET) |
| Fc gamma RI / CD64 | FRT-03 | Human Fc gamma RI / CD64 binding Kit (TR-FRET) |
| Fc gamma RIIA / CD32a | FRT-04 | Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) |
| Fc gamma RIIA / CD32a | FRT-05 | Human Fc gamma RIIA / CD32a (R167) binding Kit (TR-FRET) |
| Fc gamma RIIIA / CD16a | FRT-06 | Human Fc gamma RIIIA / CD16a (F176) binding Kit (TR-FRET) |
| Fc gamma RIIIA / CD16a | FRT-07 | Human Fc gamma RIIIA / CD16a (V176) binding Kit (TR-FRET) |
| Fc epsilon RI alpha / IgE Fc | FRT-P008 | Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET) |
| IL-4RA | FRT-P009 | Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET) |
| IL-17A | FRT-P011 | Human IL-17A / IL-17RA Inhibitor Screening Kit (TR-FRET) |
| TSLP | FRT-P012 | Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET) |
In the TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD01) is used as the donor and Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) as the acceptor. Inhibition assay of interaction of Biotinylated Human PD-1, Fc, Avi tag (Cat, No. PD1-H82F1) and Human PD-L1, HisTag (Cat. No. PD1-H5229) by Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a typical IC50 of3.358 ug/ml.
| Molecule | Cat. No. | Product Description |
|---|---|---|
| Streptavidin | FRT-TA01 | Streptavidin Protein FA-Labeled (for TR-FRET) |
| IgG | FRT-TA02 | Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) |
| IgG (H&L) | FRT-TA03 | Monoclonal Anti-Mouse IgG Antibody FA-Labeled (for TR-FRET) |
| His | FRT-TA04 | Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (for TR-FRET) |
| His | FRT-TA05 | Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (for TR-FRET) |
| Flag(M2) | FRT-TA06 | Monoclonal Anti-Flag Tag Antibody FA-Labeled (for TR-FRET) |
| MYC | FRT-TA07 | Monoclonal Anti-c-Myc Tag Antibody FA-Labeled (for TR-FRET) |
| Streptavidin | FRT-TD01 | Streptavidin Protein Europium chelate (for TR-FRET) |
| IgG | FRT-TD02 | Monoclonal Anti-Human IgG Antibody Europium chelate (for TR-FRET) |
| IgG (H&L) | FRT-TD03 | Monoclonal Anti-Mouse IgG Antibody Europium chelate (for TR-FRET) |
| Flag(M2) | FRT-TD06 | Monoclonal Anti-Flag Tag Antibody Europium chelate (for TR-FRET) |
| MYC | FRT-TD07 | Monoclonal Anti-c-Myc Tag Antibody Europium chelate (for TR-FRET) |
| / | DB-04 | TR-FRET Sample Dilution Buffer, pH7.4 |
| / | DB-05 | TR-FRET Detection Buffer, pH7.4 |
| / | DB-06 | TR-FRET Detection Buffer, pH6.0 |
| / | DB-07 | TR-FRET Detection Buffer, pH5.5 |
| / | DB-08 | TR-FRET Detection Buffer, pH5.0 |
More TR-FRET products are coming soon. Stay tuned for updates!
In response to the growing complexity of biopharmaceutical R&D, ACROBiosystems now offers specialized custom TR-FRET kit development service. Our tailored solutions empower your research with:
Whether you are developing small molecule drugs or biologics, our TR-FRET solutions provides the precision tools you need to shorten development timelines and improve data quality!
Service Workflow
| Service Stage | Deliverables | Timeline | Price |
|---|---|---|---|
| Method Development |
1. Method development data （Note: Method development data only. For method transfer, please contact us separately.） | 2-3 weeks |
Project-based, customized quotation. Contact us for a tailored plan. |
| Method Validation | 1. Method validation report | 2-3 weeks | |
| Kit Production |
1. TR-FRET kit/components 2. Quality inspection report (COA) 3. Detailed user manual (DS) | 2-3 weeks |
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