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Your Position: Casa > Gene knockout Cell Lines
Gene knockout Cell Lines
Introduction
Gene knockout cell lines are engineered using gene-editing technology to completely delete a specific gene, enabling researchers to study gene function, disease mechanisms, drug efficacy, and personalized medicine.
Among gene-editing tools, CRISPR-Cas9 stands out for its high efficiency and precision in targeted genome modifications. By designing a guide RNA (gRNA) specific to the target gene, CRISPR-Cas9 directs the Cas9 nuclease to a precise genomic location, where it induces a double-strand DNA break. The cell then repairs this break through the non-homologous end joining (NHEJ) pathway, often resulting in insertions or deletions (Indels). These mutations typically cause a reading frame shift, effectively knocking out the target gene at the endogenous genomic level.
ACROBiosystems leverages a well-established and highly efficient CRISPR-Cas9 gene-editing platform, encompassing a comprehensive workflow that includes sgRNA design and validation, gene-edited cell line development, and genomic sequencing. With a commitment to innovation and quality, ACROBiosystems continuously develops high-quality gene knockout cell lines, rigorously validated for functionality and stability. These reliable, cell-based research tools are designed to accelerate drug discovery and development, providing researchers with the precision and confidence needed to advance their work at the cellular level.
Application Scenarios

Gene Function Research [1]

Gene Function Research

Target & Transduction Research

Target & Transduction Research

Mechanism of Disease Research [2]

Mechanism of Disease Research

Molecular Targeting Drug Research [3]

Molecular Targeting Drug Research

Drug Discovery and Screening

Drug Discovery and Screening

Drug Safety Evaluation

Drug Safety Evaluation

Product Features

Rigorous Quality Control: Each cell line undergoes thorough validation, including FACS expression analysis and genomic sequencing, ensuring high reliability and consistency.

Traceable Cell Line Origin: Complete documentation provides clear lineage and history, supporting transparency and reproducibility in research.

End-to-End Support: Comprehensive pre- and after-sales assistance to address technical inquiries and ensure seamless integration into your workflow.

Regulatory Compliance: Ready-to-use regulatory documents are available to support clinical submissions and meet CMC requirements.

Roadmap

RNP method (Ultra-low off-target effects, no risk of gene integration, high editing efficiency) Recommended

RNP method

Lentiviral method (High transduction efficiency, long-term stable expression)

Lentiviral method
Contact usWith our well-established CRISPR-Cas9 gene-editing platform,Contact usContact uswe provide customized gene knockout cell lines tailored to your specific targets,
empowering your research and drug development.Contact usContact us today to take your research to the next level!Contact us
Product List
  • ADC Targeting & CAR-T Cell Therapy

MoleculeCat. No.Product DescriptionOrder/Pre-order
CD19SCRAJ-STT216Raji/Human CD19 Knockout Stable Cell Line Development Service

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Relevant Cell Line Services

Relevant Cell Line Services
Relevant Cell Line Services

References

[1]. Nieuwe methode om regulatie van genen in individuele cellen te bestuderen. Hubrecht Institute. Published 2019 Jun 17.

[2]. Plundrich D, Chikhladze S, Fichtner-Feigl S, Feuerstein R, Briquez PS. Molecular Mechanisms of Tumor Immunomodulation in the Microenvironment of Colorectal Cancer. Int J Mol Sci. 2022;23(5):2782. Published 2022 Mar 3. doi:10.3390/ijms23052782

[3]. Zhang DKY, Cheung AS, Mooney DJ. Activation and expansion of human T cells using artificial antigen-presenting cell scaffolds. Nat Protoc. 2020;15(3):773-798. doi:10.1038/s41596-019-0249-0

ACRO Quality

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