Save Product
Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Biotinylated Human PD-L1 (19-134), His,Avitag (PDL-H82E4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 134 (Accession # Q9NZQ7-1).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 16.9 kDa. The protein migrates as 19 kDa and 21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Application
PDL-H82E4 works best for experiments that test the binding between PD-L1 and candidate antibodies, such as biopanning and other relevant assays.
This product is NOT suitable for testing PD1-PDL1 binding. For this type of application, we strongly recommend you to choose PD1-H82F3 as an alternative.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human PD-L1 (19-134), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human PD-L1 (19-134), His,Avitag (Cat. No. PDL-H82E4) is more than 85% and the molecular weight of this protein is around 17-25 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human PD-L1, His,Avitag (Cat. No. PDL-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat. No. PD1-H5255) with a linear range of 2-13 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PD-L1 (19-134), His,Avitag™ (Cat. No. PDL-H82E4) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-6 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
Contact Us
Drug Candidate Licensing
