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Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
PE-Labeled Human PD-L1 (19-238) Protein, His Tag (PD1-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # Q9NZQ7-1).
Predicted N-terminus: Phe 19
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Evaluation of CAR expression
FACS analysis of PE-Labeled Human PD-L1 (19-238) Protein binding cell surface Human PD-1
5e5 of HEK293/Human PD-1 Stable Cell Line were stained with 100 μL of 1:10 dilution (10 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human PD-L1 (19-238) Protein, His Tag (Cat. No. PD1-HP2H3) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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