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Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Human PD-L1 Protein, Mouse IgG1 Fc Tag (PD1-H52A3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human PD-L1 Protein, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) is more than 95% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) at 1 μg/mL (100 μL/well) can bind Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) with a linear range of 0.008-0.25 μg/mL (QC tested).
Protocol
Serial dilutions of Nivolumab were added into Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3): Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.38426 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3) on AMC Biosensor, can bind Human Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 2.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of C4B-293T cells overexpressing PD-1 were stained with 100 μL of 10 μg/mL of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No.PD1-H52A3) and Negative Control Protein respectively, washed and then followed by PE-anti Mouse IgG1 Antibody and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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