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Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Rhesus macaque PD-1, His Tag (PD1-R52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ2-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rhesus macaque PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) at 2 μg/mL (100 μL/well) can bind nivolumab with a linear range of 0.1-6 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PD-L2, Fc,Avitag (Cat. No. PD2-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) with a linear range of 0.078-0.625 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
22 Details
- Drugs in Clinical Trials：
193 Details
- Highest Development Stage：
Approved
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