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Product Details
Synonym
TNFRSF14, ATAR, HVEA, HVEM, LIGHTR, TR2, CD270
Source
Human HVEM, Mouse IgG2a Fc Tag (HVM-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # Q92956-1).
Predicted N-terminus: Leu 39
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human HVEM, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human HVEM, Mouse IgG2a Fc Tag (Cat. No. HVM-H5257) is more than 90% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) at 5 μg/mL (100 μL/well) can bind Human HVEM, Mouse IgG2a Fc Tag (Cat. No. HVM-H5257) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human BTLA (31-150), His,Avitag (Cat. No. BTA-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Mouse IgG2a Fc Tag (Cat. No. HVM-H5257) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 14
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
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