Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CT4-C5256-200ug
$200.00
CT4-C5256-1mg (500ug X 2)
$1000.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    CTLA4, CD152

  • Source

    Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (CT4-C5256) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.

    Predicted N-terminus: Ala 37

    Request for sequence

  • Molecular Characterization

    CTLA-4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 39.9 kDa. The protein migrates as 42-50 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CTLA-4 SDS-PAGE

    Cynomolgus / Rhesus macaque CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    CTLA-4 SEC-MALS

    The purity of Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) is more than 90% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CTLA-4 ELISA

    Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-1, His Tag (Cat. No. CD0-C52H3) with a linear range of 0.039-1.25 μg/mL (QC tested).

    Protocol
  • Bridging ELISA

     CTLA-4 BRIDGING ELISA

    Validated with Bispecific Antibody.
    Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 1 μg/mL, add increasing concentrations of Cadonilimab and then add Biotinylated Cynomolgus PD-1 Protein, Fc, Avitagag (Cat. No. PD1-C82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.24 ng/mL (Routinely tested).

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Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Recent Advances

 
Drug Development Progress
  • English Name:

    Cytotoxic T-Lymphocyte-Associated Antigen 4

  • Category:

  • Approved Drugs:

    5 Details

  • Drugs in Clinical Trials:

    65 Details

  • Highest Development Stage:

    Approved

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