Product Details
Synonym
CTLA4, CD152
Source
Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (CT4-C5256) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.
Predicted N-terminus: Ala 37
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.9 kDa. The protein migrates as 42-50 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Cynomolgus / Rhesus macaque CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) is more than 90% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-1, His Tag (Cat. No. CD0-C52H3) with a linear range of 0.039-1.25 μg/mL (QC tested).
Protocol
Bridging ELISA

Validated with Bispecific Antibody.
Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (Cat. No. CT4-C5256) at 1 μg/mL, add increasing concentrations of Cadonilimab and then add Biotinylated Cynomolgus PD-1 Protein, Fc, Avitagag (Cat. No. PD1-C82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.24 ng/mL (Routinely tested).
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Background
Recent Advances
- English Name:
Cytotoxic T-Lymphocyte-Associated Antigen 4
- Category:
- Approved Drugs:
5 Details
- Drugs in Clinical Trials:
65 Details
- Highest Development Stage:
Approved



















