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Product Details
Synonym
CD28, Tp44
Source
Rat CD28, His Tag (CD8-R52H9) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Leu 150 (Accession # P31042-1).
Predicted N-terminus: Asn 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Rat CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-BLI
Loaded Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) on SA Biosensor, can bind Rat CD28, His Tag (Cat. No. CD8-R52H9) with an affinity constant of 15 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human B7-2, Fc,Avitag, premium grade (Cat. No. CD6-H82F5) on SA Biosensor, can bind Rat CD28, His Tag (Cat. No. CD8-R52H9) with an affinity constant of 6.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Phase 2 Clinical
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