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Product Details
Synonym
CD28, Tp44
Source
Mouse CD28, Fc Tag (CD8-M5258) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).
Predicted N-terminus: Asn 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse CD28, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse CD28, Fc Tag (Cat. No. CD8-M5258) is more than 95% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
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Bioactivity-BLI
Loaded Mouse CD28, Fc Tag (Cat. No. CD8-M5258) on Protein A Biosensor, can bind Mouse B7-2, His Tag (Cat. No. CD6-M52H0) with an affinity constant of 7.9 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Mouse CD28, Fc Tag (Cat. No. CD8-M5258) on Protein A Biosensor, can bind Mouse B7-1, His Tag (Cat. No. CD0-M5228) with an affinity constant of 8.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Phase 2 Clinical
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