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Product Details
Synonym
BTLA, CD272
Source
Human BTLA (31-150), Mouse IgG2a Fc Tag (BTA-H5258) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).
Predicted N-terminus: Lys 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human BTLA (31-150), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human BTLA (31-150), Mouse IgG2a Fc Tag (Cat. No. BTA-H5258) is more than 95% and the molecular weight of this protein is around 83-112 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150), Mouse IgG2a Fc Tag (Cat. No. BTA-H5258) with a linear range of 10-156 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B- and T-lymphocyte attenuator
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
6 Details
- Highest Development Stage：
Phase 3 Clinical
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