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Product Details
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
Biotinylated Mouse BCMA, Fc,Avitag (BCA-M82F0) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 49 (Accession # O88472-1).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.4 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to O-linked glycosylation which has been verified by O-Glycosidase and Neuraminidase digestion.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Mouse BCMA, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) was greater than 85% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Mouse BAFF, Mouse IgG2a Fc Tag (Cat. No. BAF-M5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) on SA Biosensor, can bind Mouse BAFF, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BAF-M5257) with an affinity constant of 3.75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) on SA Biosensor, can bind Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) with an affinity constant of 2.19 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell maturation protein
- Category：
- Approved Drugs：
8 Details
- Drugs in Clinical Trials：
145 Details
- Highest Development Stage：
Approved
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