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Product Details
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
Human BCMA (P33S) Protein, His Tag (BCA-H52H4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1 (P33S)).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.9 kDa. The protein migrates as 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human BCMA (P33S) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human BCMA (P33S) Protein, His Tag (Cat. No. BCA-H52H4) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human BCMA (P33S) Protein, His Tag (Cat. No. BCA-H52H4) at 2 μg/mL (100 μL/well) can bind Human APRIL Protein, Fc Tag (Cat. No. APL-H5263) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human BCMA (P33S) Protein, His Tag (Cat. No. BCA-H52H4) at 2 μg/mL (100 μL/well) can bind Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) with a linear range of 0.1-6 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA (P33S) Protein, His Tag (Cat. No. BCA-H52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human BCMA Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-Human BCMA Antibody, Human IgG1 captured on Protein A Chip can bind Human BCMA (P33S) Protein, His Tag (Cat. No. BCA-H52H4) with an affinity constant of 9.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell maturation protein
- Category：
- Approved Drugs：
8 Details
- Drugs in Clinical Trials：
145 Details
- Highest Development Stage：
Approved
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