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Product Details
Synonym
BAFFR, TNFRSF13C, BROMIX, CD268, CVID4, prolixin, BAFF-R
Source
Human BAFFR Protein, Fc Tag (BAR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 33.1 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human BAFFR Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5253) is more than 95% and the molecular weight of this protein is around 65-90 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 5 μg/mL (100 μL/well) can bind Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5253) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Immobilized Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5253) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-BAFFR Antibody, Human IgG1 immobilized on CM5 Chip can bind Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5253) with an affinity constant of 9.66 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5253) captured on Protein A Chip can bind Human BAFF, His Tag (Cat. No. BAF-H5248) with an affinity constant of 129 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell activating factor receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
14 Details
- Highest Development Stage：
NDA/BLA
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