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Product Details
Synonym
TNFSF13B, BAFF, BLYS, CD257, DTL, TALL1, THANK, TNFSF20, ZTNF4, TALL-1
Source
Biotinylated Human BAFF Protein, His,Avitag (BAF-H82Q2) is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.2 kDa. The protein migrates as 21-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human BAFF Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) is more than 95% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Immobilized Human TACI Protein, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human BCMA, His Tag (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-BCMA Neutralizing Antibody, Mouse IgG1 (Clone: AM43, Cat. No. BCA-M43) and 0.01 μg/mL (100 μL/well) Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) with a half maximal inhibitory concentration (IC50) of 14.79 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258): Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 1.1323 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag: Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.7363 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) on SA Chip can bind Human TACI Protein, Fc Tag with an affinity constant of 1.35 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) on SA Chip can bind Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) on SA Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell-activating factor/B lymphocyte stimulator
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Approved
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