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Product Details
Synonym
CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Source
Rat B7-1, His Tag (B71-R52H9) is expressed from human 293 cells (HEK293). It contains AA Ile 39 - Gln 248 (Accession # Q62680-1).
Predicted N-terminus: Ile 39
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rat B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rat B7-1, His Tag (Cat. No. B71-R52H9) is more than 85% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rat CTLA-4, Fc Tag (Cat. No. CT4-R5259) at 5 μg/mL (100 μL/well) can bind Rat B7-1, His Tag (Cat. No. B71-R52H9) with a linear range of 0.002-0.05 μg/mL (QC tested).
Protocol
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Background
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Recent Advances
Drug Development Progress- English Name：
Cluster of differentiation 80
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
10 Details
- Highest Development Stage：
Approved
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