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Materials Provided
IDComponentsSizeEP157-C01High-bind Plate1 plateEP157-C02Mouse PD-L125 μgEP157-C03Biotinylated Mouse PD-130 μgEP157-C04Anti-Mouse PD-L1 Neutralizing Antibody20 μgEP157-C05Streptavidin-HRP5 μgEP157-C06Coating Buffer12 mLEP157-C0710xWashing Buffer50 mLEP157-C08Blocking Buffer50 mLEP157-C09Substrate Solution12 mLEP157-C10Stop Solution7 mLProduct Overview
Mouse PD-L1 : Mouse PD-1 [Biotinylated] Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human Mouse PD-L1 binding to Mouse PD-1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human Mouse PD-L1 binding to Mouse PD-1.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF Mouse PD-L1: Mouse PD-1 [Biotinylated] BINDING BY ANTI-Mouse PD-L1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-Mouse PD-L1 Neutralizing antibody (Catalog # EP157-C04) (1:1 serial dilution, from 5 μg/mL to 0.00244μg/mL) was added into Mouse PD-L1: Biotinylated Mouse PD-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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